Journal article
Regulatory T cells participate in CD39-mediated protection from renal injury
YM Wang, JL Mcrae, SC Robson, PJ Cowan, GY Zhang, M Hu, T Polhill, Y Wang, G Zheng, Y Wang, VWS Lee, RJ Unwin, DCH Harris, KM Dwyer, SI Alexander
European Journal of Immunology | WILEY | Published : 2012
Abstract
CD39 is an ecto-enzyme that degrades extracellular nucleotides, such as ATP, and is highly expressed on by the vasculature and circulating cells including Foxp3+ regulatory T (Treg) cells. To study the role of purinergic regulation in renal disease, we used the adriamycin nephropathy (AN) mouse model of chronic renal injury, using human CD39-transgenic (hCD39Tg) and wild-type (WT) BALB/c mice. Effects of CD39 expression by Treg cells were assessed in AN by adoptive transfer of CD4+CD25+ and CD4+CD25- T cells isolated from hCD39Tg and WT mice. hCD39Tg mice were protected from renal injury in AN with decreased urinary protein and serum creatinine, and significantly less renal injury compared w..
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Grants
Awarded by National Institute of Allergy and Infectious Diseases
Funding Acknowledgements
This work was supported by research grants from the National Health and Medical Research Council of Australia (NHMRC grant 1030463, 571343, and 632519) and HL094400 NIH USA. We thank the animal house staff in the Westmead Hospital Animal House Facility for care of the animals. Thanks to Fredrick Tam (Imperial College), Anthony JF d'Apice (St. Vincent's Hospital), and Dr Huiling Wu (University of Sydney, Australia) for advice. We thank, Jimmy Jianheng Zhou (Children's Hospital at Westmead), SoRa Yes Lee, Thian Kui Tan, and Tim Tzu-Ting Hsu (Westmead Hospital, Australia) for technical assistances.